T98G is a glioblastoma cell line used in brain cancer research and drug development. The T98G cell line was derived from a 61-year-old human male and has a hyperpentaploid chromosome count with a modal number ranging from 128 to 132. The cells are not tumorigenic in mice, but do proliferate with proper anchorage in cell culture. T98G cells are known for having high expression of the ACTA2 gene, which is involved in cell motility and structure. T98G cells are poly… WebApr 16, 2024 · Indeed, the p53 pathway has been found to be altered in 87% of GB cases ( 6 ), by either mutations/deletions of the TP53 gene itself ( 6, 7) or defects affecting other members of the pathway, such as the amplification of the two main p53 negative regulators, murine double minute (MDM)2 and MDM4, and mutations/deletions of the cyclin …
Temozolomide resistance in glioblastoma multiforme - ScienceDirect
WebIndeed, T98G is p53 mutation, has a positive MGMT status, and is resistant to TMZ . Only U251 and U87 have been reported to contain a CSC population, but these are both MGMT negative. Moreover, LN229 and U251 are different from T98G and U87 by expressing nestin, SOX2, and CD44 . Then, the R2J cell line really has added value because in addition ... WebWe examined sensitivity to neutron irradiation and TMZ in two glioblastoma cell lines: T98G, which is p53-mutant with high levels of MGMT activity; and A172, which is p53-wild … meaning of abash
(PDF) Handbook of p53 mutation in cell lines - ResearchGate
WebExogenously overexpressed miR-3928 leads to the upregulation of wild-type p53 in GBM cell lines A172 and U87 that have wild-type p53 (*) but not in T98G and U251 cell lines, which have mutant p53. ( b ) With immunoblots showing that miR-3928 overexpression induces p53 phosphorylation in A172 cells, UV treated cells are used as positive control ... WebAug 28, 2008 · In T98G cells, high 53BP1 foci numbers (≥10 foci per cell) were predominantly observed in BrdUrd + bystander cells ( Fig. 4C ), confirming a link between S-phase/DNA replication and the early bystander response. WebIn T98G (p53 mut) cells, we did not observe an induction of G1 checkpoint mediators, but DY induced phosphorylation of histone H3 at serine 10, previously shown to be a specific phosphorylation site during prophase and important for chromatin condensation. 30,31 These data suggest DY induces a cell cycle arrest specifically in mitosis in p53 … peas seeds for sale