Promega triplex assay
WebPCR Basics. PCR, the polymerase chain reaction, is a core technique that has revolutionized molecular biology. In PCR, a DNA molecule is targeted and copied multiple times, allowing … WebThe ApoTox-Glo triplex assay (Promega, G6320) is more complex and is centered on the proteolytic action of biomarkers present in the cell. It was used to evaluate cell viability, cell cytotoxicity, and caspase 3/7 activation (a marker of apoptosis) 24, 48 or 72 h …
Promega triplex assay
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WebApr 11, 2024 · The ApoTox-Glo Triplex Assay (Promega, Madison, WI, USA) was used to measure live-cell protease activity, dead-cell protease. activity, and caspase-3/7 activity a s markers of viability, cytotoxicity, WebJun 15, 2011 · • Promega has had great success in combining cell-based assays with fluorescent and luminescent readouts. The multiplex assay format typically begins with …
WebTriplex Assay Sensitivity A pool of Jurkat cells was divided into two fractions. One fraction was treated to simulate cytotoxicity, whereas the other was left untreated. The Caspase- Glo 3/7 Assay can detect caspase 3/7 activation at lower levels than fluorescent methods. Detect caspase- 3/7 activation earlier and with fewer cells. Viability Assay WebThe ApoTox-Glo™ Triplex Assay combines three Promega assay chemistries to assess viability, cytotoxicity and caspase activation events within a single assay well. The first …
WebPromega’s P450-Glo™, ONE-Glo™, and CellTiter-Fluor™triplex assay provides a straightforward way to assess multiple compound affects a compound may have on a … WebApoTox-Glo™ Triplex Assay G6320 10ml G6321 5 x 10ml ApoTox-Glo is a trademark and Caspase-Glo is a registered trademark of Promega Corporation. Products may be covered …
WebApoTox-Glo™ Triplex Assay with suspension Jurkat cells treated with staurosporine. Staurosporine treatment for 6 hours results in a dose-dependent decrease in viability and …
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