Webblimpet hemocyanin (KLH) Ag into the hind footpads of WT B6 mice. Five days later, the extent of in vivo Ag presentation was examined by assessing IL-2 secretion and proliferation of T cells obtained from the draining popliteal lymph nodes as defined by a recall response to KLH. In this assay, the precursor frequency of WebbIntraepidermal nerve fibers (IENF) of the hind footpads were identified by protein gene product (PGP) 9.5 immunohistochemistry. The peptidergic IENF were determined by …

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Webb1 aug. 2000 · Here we injected CpG-DNA into the hind footpads of mice, waited 7 days, and then inoculated the hind footpads with either liposome-encapsulated OVA (Lipo-OVA) or soluble OVA. Lipo-OVA induced mild CTL activity in non-DNA-sensitized mice (Fig. 3,A). However, the response was greatly enhanced if the mice were “presensitized” with CpG … Webb15 aug. 2010 · Hind footpads were measured 10 days later then challenged with an injection of Candida antigen. DTH was measured by footpad swelling 24 hours post-challenge. Negative controls were measured in mice not immunized but challenged. Positive controls were non-irradiated, but immunized and challenged mice. N > 5 per … memphis bed and breakfast beale street

Pain threshold changes in adjuvant-induced inflammation: a

WebbA chronic hyperalgesic condition was induced in mice by the injection of Freund's complete adjuvant (FCA) into the lower lumbar region or directly into the hind footpads. … Webb29 maj 2024 · Hind footpads of mice were disinfected and homogenized in PBS (−) by using a glass homogenizer. The resultant homogenate was subjected to serial 10-fold dilutions with normal saline and 0.1 mL ... WebbThe other side of the hind footpads were subcutaneously injected with 100 μl saline as a control. a Foot thickness was measured at the indicated time points after injection into … memphis belle abc promo 1994