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WebJun 13, 2016 · Briefly, tissues were incubated in blocking buffer solution for one hour (5% Goat Serum (Sigma G6767), 1% BSA (Fisher BP1600-100), 0.05% Sodium Azide-20% (RPI, S24080-500), 0.5% Triton X-100 (Fisher BP151-100), 1X PBS-10X, dH2O). Tissues were incubated overnight with appropriate primary antibodies diluted in blocking buffer, … WebOct 19, 2015 · Membranes were then blocked in 5% (wt/vol) nonfat dry milk or 5% (wt/vol) BSA (Fisher; BP1600) in Tris-buffered saline + 0.05% Tween 20 (TBST) for 1 h. After blocking, membranes were incubated overnight at room temperature with the appropriate primary antibody. WebFeb 25, 2024 · Samples were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15 710) for 15 min at room temperature, washed twice with 3% bovine serum albumin (BSA; Fisher, BP1600) in phosphate-buffered saline (PBS) and permeabilized with 0.5% Triton X-100 (Sigma, T8787) in PBS for 20 min. Samples were then washed twice … criterion hotel menu