Bulked segregant analysis bsa dna sequencing
WebFeb 13, 2024 · With the release of sequenced genomes, the combined application of bulked segregant analysis (BSA) and next-generation sequencing technology represents a … WebBulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked …
Bulked segregant analysis bsa dna sequencing
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WebApr 11, 2024 · A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with … WebBulked segregant analysis (BSA) has been used to map many agronomical traits in several crops, including resistance genes to fungal pathogens. Using BSA in diploid …
WebNov 25, 2024 · Therefore, this study aimed to identify significant QTLs associated with NCLB by utilizing next-generation sequencing-based bulked-segregant analysis (BSA). Parental lines GML71 (resistant) and Gui A10341 (susceptible) were used to develop segregating population F 2. Two bulks with 30 plants each were further selected from the … WebNov 6, 2024 · Here, we describe a bulked-segregant analysis sequencing (BSA-Seq) protocol for cloning mutant genes in maize. Our simple strategy can be used to quickly …
WebMay 7, 2012 · Bulked segregant analysis (BSA) is an efficient method to rapidly and efficiently map genes responsible for mutant phenotypes. BSA requires access to quantitative genetic markers that are polymorphic in the mapping population. WebJan 1, 2024 · Combining next-generation sequencing (NGS) and bulked-segregant analysis (BSA) [, ... About 5 μg of DNA from each bulk was used for the next generation …
WebJan 20, 2024 · The BSA-NGS analysis revealed that two candidate loci from 1.7 to 2.0 Mbp and 5.6 to 6.2 Mbp on chromosome (Chr) 9 were associated with dwarfism. We validated these SNPs in F2 progeny using a high-resolution melting assay. Based on the results, we identified a perfect cosegregation region of 1,872,994 to 1,866,655 bp on Chr 9.
WebQTL mapping is a fundamental approach for understanding the genetic inheritance of DM resistance in cucumber. Recently, many studies have reported that a combination of … fire tv stick willhaben wienWebBulk segregation analysis (BSA) utilizes a strategy of pooling individuals with extreme phenotypes to conduct economical and rapidly linked marker screening or quantitative … ets 2 mods flowerWebJan 28, 2024 · Key message Using bulked segregant analysis of exome sequence, we fine-mapped the ABA-hypersensitive mutant ERA8 in a wheat backcross population to … ets2 mods scania 143mWebTwo bulks with 30 plants each were further selected from the segregating population for sequencing along with the parental lines. High throughput sequencing data was used for BSA. We identified 10 QTLs on Chr 1, Chr 2, Chr 3, … ets 2 mods scania hooklift 1.43WebApr 11, 2024 · A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype. ... followed by a bulked segregant analysis (BSA) using whole genome … ets2 mods scania busWebSequencing this stretch of DNA identified the central portion as the putative Mat1-2 locus of M graminicola. ... NGS-based mapping approaches using bulked segregant analysis … ets 2 mods mercedes actros 2019Web2 days ago · The enormous amount of short reads generated by the new DNA sequencing technologies call for the development of fast and accurate read alignment programs. ... ets 2 mods scania 143